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Amplification examination laboratory is generally divided into four separate working areas: reagent storage and preparation area, sample preparation area, amplification reaction mixture preparation and amplification area and amplified product analysis area. In order to avoid cross contamination, all working areas must be entered in a single direction.
擴增檢驗實驗室原則上分為四個單獨的工作區(qū)域：試劑貯存和準備區(qū)、標本制備區(qū)、擴增反應混合物配制和擴增區(qū)、擴增產(chǎn)物分析區(qū)。為避免交叉污染，進入各個工作區(qū)域必須嚴格遵循單一方向進行。

Reagent preparation area→ sample preparation area → amplification area→product analysis area

試劑準備區(qū)→樣品制備區(qū)→擴增區(qū)→產(chǎn)物分析區(qū)。

Reagents and samples between each experimental area should be passed through the window. 

各實驗區(qū)之間的試劑及樣品傳遞應通過傳遞窗進行。

Reagent preparation area / 試劑準備區(qū)  

The main operations of the reagent preparation area include preparation/packaging of reagents and main reaction mixture preparation. Reagents and materials used for sample production shall be directly transferred to this area without going through other areas. Reagent raw materials must be stored in this area and prepare the required storage reagents in this area. As for air pressure control, no strict requirements are required.
該實驗區(qū)主要進行的操作為貯存試劑的制備、試劑的分裝和主反應混合液的制備。試劑和用于樣品制作的材料應直接運送至該區(qū)，不得經(jīng)過其他區(qū)域。試劑原材料必須貯存在本區(qū)內(nèi)，并在本區(qū)內(nèi)制備成所需的貯存試劑。 對與氣流壓力的控制，本區(qū)并沒有嚴格的要求。

Sample preparation area / 標品制備區(qū)  

The main operations in the area include sample preservation, nucleic acid (RNA, DNA) extraction, storage and addition to amplification reaction tube and determination of DNA synthesis. 
該區(qū)域主要進行的操作為樣本的保存、核酸(RNA、DNA)提取、貯存及其加入至擴增反應管和測定DNA的合成。
The pressure gradient in this area is required to be positive pressure relative to the adjacent area.  in order to avoid aerosol pollution from the adjacent area. In addition, due to the pollution caused by aerosol in the sample operation, it is necessary to avoid unnecessary movement in this area.
本區(qū)的壓力梯度要求為：相對于鄰近區(qū)域為正壓，以避免從鄰近區(qū)進入本區(qū)的氣溶膠污染。另外，由于在加樣操作中可能會發(fā)生氣溶膠所致的污染，所以應避免在本區(qū)內(nèi)不必要的走動。

Amplification area / 擴增區(qū)  

The main operations in this area include DNA amplification. In addition, addition of prepared DNA template (from sample preparation area) and preparation of reaction mixture from main reaction mixture (from reagent storage and preparation area) can also be carried out in this area.
該區(qū)域主要進行的操作為DNA擴增。此外，已制備的DNA模板(來自樣本制備區(qū))的加入和主反應混合液(來自試劑貯存和制備區(qū))制備成反應混合液等也可在本區(qū)內(nèi)進行。
The pressure gradient in this area is required to be negative pressure relative to the adjacent area in order to avoid leakage of aerosol from this area. In order to avoid pollution caused by aerosol, it is necessary to avoid unnecessary movement in this area. Individual operation such as sample addition should be carried out on super clean table.
本區(qū)的壓力梯度要求為：相對于鄰近區(qū)域為負壓，以避免氣溶膠從本區(qū)漏出。為避免氣溶膠所致的污染，應盡量減少在本區(qū)內(nèi)的不必要的走動。個別操作如加樣等應在超凈臺內(nèi)進行。

Product analysis area / 產(chǎn)物分析區(qū)  

The main operations in this area include determination of amplified fragments.
該區(qū)域主要進行的操作為擴增片段的測定。
This area is the main source for amplification product contamination, so the pressure gradient in this area is required to be negative pressure relative to the adjacent area in order to avoid amplification product from passing from this area to other areas.
本區(qū)是最主要的擴增產(chǎn)物污染來源，因此對本區(qū)的壓力梯度的要求為：相對于鄰近區(qū)域為負壓，以避免擴增產(chǎn)物從本區(qū)擴散至其它區(qū)域。